Sony Biotechnology Inc. Sony Biotechnology Inc.cartUS
SI8000 Cell Motion Imaging System
Applications
Specifications

SI8000 Cell Motion Imaging System
SI8000 Cell Motion Imaging System
  • High speed video recording that provides a deeper depth of data
  • Noninvasive label free motion analysis that provides data precision
  • User-friendly software that provides high temporal and spatial resolution for greater depth and precision of data
  • Measures the actual movement of cells instead of inferring motion from electrophysiology, impedance, or invasive dyes

The Sony SI8000 Live Cell Imaging System quantifies cellular motion in a noninvasive label-free environment. Leveraging Sony expertise and patented imaging technologies, the SI8000 uses high speed video imaging with a unique motion vector analysis algorithm to capture the motion of cells with high temporal and spatial fidelity. These technologies and knowhow deliver results that offer greater depth and precision.

Using the integrated phase contrast microscope and stage top incubator, cells can be visualized in a humidity and climate controlled environment. High-resolution data including high speed cardiomyocyte contractility, and cellular migration, is captured by the system.

Unlike other systems where motion is inferred from impedance or electrophysiological measures, the SI8000 records actual cellular motion, delivering more precise information that is less susceptible to false negatives and false positives.

High Speed Motion Capture, Quantification and Visualization

The Sony SI8000 system is equipped with a phase contrast microscope, a high-speed charge-coupled device (CCD) video camera, CO2-incubator and sophisticated easy-to-use software.

Focus and light conditions are automated through the SI8000 software. Video imaging captures activity at a high frame rate (up to 150 fps) to deliver deep data rich results. Displacement and magnitude of cellular motion are calculated using a Motion Vector algorithm developed by Sony for HD television. This allows researchers to accurately analyze movement of single cell or cell clusters such as monolayers continuously for extended periods of time. Precise detection of cellular motion provides label-free analysis of cardiomyocyte contractility, cellular migration, and apoptosis assays without the need to infer data from electrophysiological, impedance, or dye based assays.

Image Acquisition

The software automatically detects and adjusts focus based on luminance and contrast conditions. Automation in the motorized stage allows for high throughput analysis of multi-well culture plates.

Motion Vector Analysis

High spatial and temporal resolution enables high fidelity detection of the motion of cells (red arrow). The analysis software searches for luminance similarity using the block matching method from one frame to the next to accurately calculate displacement vectors, quantifying the distance the cells moved for richer and more precise data.

Quantification and Visualization

The SI8000 is label-free so cells can be observed in their natural state free from outside interactions from potentially toxic dyes or fluorochromes. This capability is particularly useful for toxicity and safety pharmacology studies as it allows researchers to see how cells behave as a drug is introduced. An optional CO2 incubator reliably manages an optimal environmental for in-vitro cell observation.

The scanning stage controls the X, Y and Z position of the imaging system for precise and repeatable positioning and re-positioning, supporting high throughput analysis.

Data analysis software enables researchers to quantify and visualize cells in motion. Cardiomyocytes can be quantified using contraction and relaxation speed, average deformation distance, as well as contraction and relaxation duration. Power Spectral Density that measures the strength of motion in the frequency domain is also supported. Graphic outputs include heat maps of contraction, relaxation, propagation and isochrone maps that detect abnormalities in cardiac cells as well as cellular and sub-cellular motion visualization.

Quantification


In Cardiomyocytes, a beat profile is obtained from Regions of Interest (ROIs). ROIs can be positioned over single cells or clusters of cells. From the beat profile, contraction and relaxation parameters can be calculated. A sophisticated Motion Vector algorithm allows for highly accurate propagation analysis and quantification for a richer and deeper understanding of results.


Overlay of the beat profiles showing contraction (first peak) and relaxation (second peak). The high speed camera enables precise detection of both contraction and relaxation of a beating cardiomyocyte for more precise indication of beat parameters.


A series of beat profiles showing contraction (first peak) and relaxation (second peak). By measuring both contraction and relaxation, the SI8000 provides a complete profile of the actual contraction unlike other systems that infer contraction parameters from other measures.

Visualization



The sophisticated Motion Vector algorithm yields precise visualization of motion parameters. Power spectral density (PSD) analysis provides visualization of the speed of motion in single cells or across cell cultures. PSD is displayed in Cardiomyocytes (left) and neuron culture (right.)

Quantification of cardiomyocytes contractility for Safety Pharmacology assays

High speed video imaging analyzes cardiomyocyte contraction to detect subtle changes that can occur in response to compounds. The system delivers excellent insights for safety pharmacology assays. The phase contrast microscope and high speed video imaging improves spatial resolution of cardiomyocytes over systems that use fluorescent or voltage sensitive dyes while providing precise temporal resolution similar to electrophysiological assays. High spatial and temporal resolution offers greater depth of data and more precision for safety pharmacology assays. By analyzing the actual contraction of cardiomyocytes, the SI8000 has the ability to detect subtle changes that may occur in response to a compound or drug.


The SI8000 supports a variety of beat parameters including beat rate and interval, contraction and relaxation velocity, shortening length (displacement), contraction-relaxation duration, synchronicity of contraction, propagation of contraction, orientation of contraction direction.


This data shows simultaneous acquisition of multi-electrode array recordings (blue trace) and motion imaging (red trace). The red trace shown an Early After Depolarization (EAD) phenomenon in response to 100 nM E-4031. The SI8000 can reliably and accurately detect arrhythmic events such as EADs offering a true representation of the data.

Easily detect markers of arrhythmia

The SI8000 detects both contraction and relaxation to detect subtle differences in the beat profile that may be missed by other systems. For example, if field potential duration is modified by a drug or compound, the system determines the precise effect a drug or compound has on contraction, relaxation, or both contraction and relaxation, to deliver more precise data for analysis.

The graphs below represent the beat profile of stem cell derived cardio-myocytes in response to reference compounds that are known to have an influence on field potential duration. By analyzing both contraction and relaxation, the SI8000 can identify changes in the beat profile even in the absence of changes in electrophysiology or changes in the field potential waveforms.

The data above shows how the application of compounds can modify the beat profile. For example, (A) shows changes in the beat profile in response to lidocaine, (B) shows the beat profile in response to E-4031, (C) shows the beat profile in response to Flecainide and (D) to Astemizole. The subtle changes displayed in beat profiles elucidate the depth of data that can be achieved with the SI8000 offering a deep and precise representation of cardiomyocyte function.