With the FP7000 Cell Sorter, researchers can choose from over 44 fluorochromes** for high-parameter experiments, enabling confident sorting of complex populations using hierarchical gating strategies.

Available laser options include blue (488 nm), red (637 nm), violet (405 nm), yellow-green (561 nm), ultraviolet (349 nm), and deep ultraviolet (320 nm). Each excitation laser is optimized to deliver high performance for detecting dim and rare markers.

Detection of autofluorescence from any laser, with the ability to assign it as an independent parameter for sorting or to unmix it from fluorescence signals, further enhances data quality.

The small particle detection option enables light scattered from the 405-nm laser to be collected to detect submicron particles 100 nm in size, allowing researchers to sort them for a variety of downstream assays.

The fluidics system of the FP7000 Spectral Cell Sorter is designed to deliver high uptime and reliable performance for seamless operation. The system uses a quartz cuvette flow cell that is fixed in alignment with the lasers. The flow cell is gel-coupled to the collection optics and supports low acceleration of particles as they pass through the beam, ensuring maximum light collection for optimal sensitivity and resolution.

The FP7000 features a new O-ring-free nozzle mounted on a base plate that fits precisely under the flow cell. Each nozzle can be easily exchanged, supporting robust operation throughout the day and enabling seamless transitions between users.

Sheath excitation technology ensures reliable operation across different nozzle types and generates stable droplets across a frequency range of 12 kHz to 100 kHz for sorting into tubes and plates.

Users scan the QR code on the nozzle assembly to initiate automated startup. Automated steps of daily QC ensure best optics alignment, laser delay calculation and voltage standardization. Automated sort setup calibrates the droplets, drop delay and side streams for successful 6-way sorting.

Each nozzle can be easily exchanged, supporting robust operation over the course of the day and seamless transition between users.

The FP7000 Spectral Cell Sorter software incorporates guided workflows and intuitive tools that enable researchers generate high-quality unbiased data and superior sort results while completing tasks in a timely manner.

Experiment Designer allows users to create a new experiment using new or pre-existing spectral references.

Experiments can be created using a previous template or new template depending on the user’s needs. The experiment workspace displays plots, gates and statistics required for the setup of an experiment. Data acquisition dashboard provides access to software options where users can specify flow rate, record conditions and stop count. The sorting dashboard provides options such as choice of sorting devices, sort modes and stop count for each stream of a multi-way sorting experiment. Tools for creating plate template and adjusting plate coordinates can also be viewed here. Stream monitoring capability such sort stream and side stream camera views are displayed.